How many mL of bacteriostatic water do I add to a peptide vial?

Most researchers add 1–2mL per standard 5mg vial. Less water produces a higher concentration with fewer units per injection. More water gives a lower concentration with more units. The right amount is whatever yields a practical, measurable dose on your syringe. The calculator pre-fills each peptide's standard volume.

How do I know how much water to put in my peptide?

Choose a volume that results in drawing between 5–50 units per injection on a U-100 syringe — a range that's easy to measure accurately. For low-dose peptides under 500mcg, 1–2mL typically works. For high-dose peptides like TB-500 at 2,500mcg, 2–3mL is more practical. Use the calculator to preview results before reconstituting.

What is the difference between bacteriostatic water and sterile water for peptides?

Bacteriostatic water contains 0.9% benzyl alcohol as a preservative, inhibiting bacterial growth and allowing safe multi-dose use for up to 28 days after opening. Sterile water has no preservative and is single-use only. For research peptide protocols involving repeated injections from one vial, bacteriostatic water is the correct choice.

How do I read an insulin syringe for peptide dosing?

On a U-100 syringe, 100 units = 1mL, so 1 unit = 0.01mL. Draw back the plunger until the rubber stopper's flat edge aligns with your target unit number. The syringe calculator diagram above shows exactly which line to draw to. For small doses, use a 0.3mL syringe (30 units maximum) for better precision.

What does it mean to reconstitute a peptide?

Reconstitution dissolves a lyophilised (freeze-dried) peptide powder into a liquid diluent, typically bacteriostatic water, to create an injectable solution. Peptides are shipped as dry powder for stability. Reconstituting produces a liquid at a known concentration that can be drawn into a syringe and injected. The concentration depends on how much water you add.

Can I use sterile water instead of bacteriostatic water?

Yes, but only for a single dose — use the reconstituted solution immediately and discard any remainder. Sterile water contains no antimicrobial preservative, so repeated-access vials risk bacterial contamination. For any research protocol requiring multiple injections from one vial over days or weeks, bacteriostatic water is the correct choice.

How many units should I draw for my peptide dose?

Units (U-100) = (desired dose in mcg ÷ concentration in mcg/mL) × 100. Example: 250mcg dose, concentration 2,500mcg/mL → 250 ÷ 2,500 × 100 = 10 units. The calculator above computes this instantly for any combination of peptide, vial size, water volume, and dose. It also adjusts the result for U-50 and U-40 syringe types.

What happens if I shake a peptide vial instead of swirling it?

Vigorous shaking subjects the peptide to mechanical shear forces and creates foam, both of which break the molecular bonds that define a peptide's 3D structure. Denatured peptides lose biological activity. Always swirl the vial gently in a slow circular motion until the powder dissolves. If foam forms, let the vial stand undisturbed for 5–10 minutes before using.

How do I reconstitute a 10mg peptide vial?

Add 2–4mL of bacteriostatic water slowly down the inner vial wall. With 2mL you get 5,000mcg/mL; with 4mL you get 2,500mcg/mL. Swirl gently until fully dissolved. Label with the date and concentration. Use the calculator above to preview how many units to draw for your dose before deciding on the water volume.

What syringe should I use for peptide injections?

Use a U-100 insulin syringe for subcutaneous peptide injections. The 0.3mL syringe is ideal for small doses (under 30 units) due to its fine 1-unit increments. The 0.5mL syringe handles most standard doses. The 1mL syringe is appropriate for larger GLP-1 doses. Always use a fresh syringe for each injection.

Tirzepatide Dosage & Reconstitution Calculator

The Tirzepatide reconstitution calculator shows your exact syringe units for each dose escalation step. Enter your vial size and bacteriostatic water to get your weekly schedule.

How it works Dosage chart Peptide guides FAQ

For research and educational purposes only. Not medical advice.

Quick select

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BAC water mL

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Select a peptide above to see suggested doses

How to Calculate Peptide Reconstitution in 3 Steps

Reconstitution math uses one formula: divide the peptide mass by the water volume to get concentration, then divide your dose by that concentration to get injection volume.

Calculate concentration

Concentration (mcg/mL) = Vial size (mg) × 1,000 ÷ BAC water (mL)

Example: 5mg vial + 2mL BAC water = 5,000 mcg ÷ 2 mL = 2,500 mcg/mL

Calculate injection volume

Volume (mL) = Desired dose (mcg) ÷ Concentration (mcg/mL)

Example: 250mcg dose ÷ 2,500 mcg/mL = 0.1 mL

Convert to syringe units

Units (U-100) = Volume (mL) × 100

Example: 0.1 mL × 100 = 10 units — draw to the 10-unit line on a U-100 syringe

The formula at a glance

Units = (Dose in mcg ÷ (Vial mg × 1,000 ÷ BAC water mL)) × 100

BPC-157 example: (250 ÷ (5 × 1,000 ÷ 2)) × 100 = 10 units

What is concentration and why does it matter?

Concentration tells you how many micrograms of peptide are in each millilitre of solution. A higher concentration (less water) means you draw fewer units per dose, which some researchers prefer for subcutaneous injection comfort. A lower concentration (more water) is easier to measure precisely on a syringe. There is no universally correct concentration — use whatever results in a practical, measurable dose for your protocol.

Peptide Reconstitution Chart: Pre-Calculated Common Doses

This table shows standard doses and U-100 syringe units for all 47 peptides in our database, calculated from default vial sizes and BAC water volumes. Click any peptide name to open it in the calculator.

Peptide	Vial	BAC Water	Concentration	Standard Dose	U-100 Units
BPC-157	5 mg	2 mL	2,500 mcg/mL	250 mcg	10
TB-500	5 mg	2 mL	2,500 mcg/mL	2,500 mcg	100
Ipamorelin	2 mg	2 mL	1,000 mcg/mL	200 mcg	20
CJC-1295 (no DAC)	2 mg	2 mL	1,000 mcg/mL	100 mcg	10
CJC-1295 (with DAC)	2 mg	2 mL	1,000 mcg/mL	2,000 mcg	200
AOD-9604	5 mg	2 mL	2,500 mcg/mL	300 mcg	12
Semax	15 mg	2 mL	7,500 mcg/mL	300 mcg	4
Selank	15 mg	2 mL	7,500 mcg/mL	250 mcg	3.3
Epithalon	10 mg	2 mL	5,000 mcg/mL	10,000 mcg	200
GHK-Cu	50 mg	2 mL	25,000 mcg/mL	2,000 mcg	8
PT-141 / Bremelanotide	10 mg	2 mL	5,000 mcg/mL	1,000 mcg	20
Tesamorelin	1 mg	2 mL	500 mcg/mL	2,000 mcg	400
DSIP / Delta Sleep Inducing Peptide	5 mg	2 mL	2,500 mcg/mL	200 mcg	8
KPV	5 mg	2 mL	2,500 mcg/mL	500 mcg	20
5-Amino-1MQ	100 mg	2 mL	50,000 mcg/mL	50,000 mcg	100
Retatrutide	10 mg	2 mL	5,000 mcg/mL	4,000 mcg	80
Tirzepatide	10 mg	2 mL	5,000 mcg/mL	5,000 mcg	100
Semaglutide	5 mg	2 mL	2,500 mcg/mL	1,000 mcg	40
Melanotan 2	10 mg	2 mL	5,000 mcg/mL	500 mcg	10
Thymosin Alpha-1	1.6 mg	2 mL	800 mcg/mL	1,600 mcg	200
NAD+	500 mg	2 mL	250,000 mcg/mL	500,000 mcg	200
GHRP-2	5 mg	2 mL	2,500 mcg/mL	100 mcg	4
GHRP-6	5 mg	2 mL	2,500 mcg/mL	100 mcg	4
Hexarelin	2 mg	2 mL	1,000 mcg/mL	200 mcg	20
MK-677	25 mg	2 mL	12,500 mcg/mL	15,000 mcg	120
Sermorelin	3 mg	2 mL	1,500 mcg/mL	300 mcg	20
IGF-1 LR3	1 mg	2 mL	500 mcg/mL	40 mcg	8
PEG-MGF	2 mg	2 mL	1,000 mcg/mL	300 mcg	30
Follistatin 344	1 mg	2 mL	500 mcg/mL	100 mcg	20
Oxytocin	2 mg	2 mL	1,000 mcg/mL	20 mcg	2
LL-37	5 mg	2 mL	2,500 mcg/mL	200 mcg	8
Dihexa	5 mg	2 mL	2,500 mcg/mL	1,000 mcg	40
SS-31	50 mg	2 mL	25,000 mcg/mL	10,000 mcg	40
Humanin	5 mg	2 mL	2,500 mcg/mL	1,000 mcg	40
MOTS-c	10 mg	2 mL	5,000 mcg/mL	5,000 mcg	100
Gonadorelin	2 mg	2 mL	1,000 mcg/mL	100 mcg	10
Kisspeptin-10	5 mg	2 mL	2,500 mcg/mL	100 mcg	4
MT-1 (Melanotan 1)	10 mg	2 mL	5,000 mcg/mL	1,000 mcg	20
VIP (Vasoactive Intestinal Peptide)	5 mg	2 mL	2,500 mcg/mL	100 mcg	4
Snap-8	100 mg	2 mL	50,000 mcg/mL	0 mcg	0
Argireline	100 mg	2 mL	50,000 mcg/mL	0 mcg	0
Leuphasyl	100 mg	2 mL	50,000 mcg/mL	0 mcg	0
Palmitoyl Tripeptide-1	100 mg	2 mL	50,000 mcg/mL	0 mcg	0
GHK (Gly-His-Lys)	50 mg	2 mL	25,000 mcg/mL	0 mcg	0
Copper Peptide GHK-Cu 3%	500 mg	2 mL	250,000 mcg/mL	0 mcg	0
TB4-Frag	5 mg	2 mL	2,500 mcg/mL	1,000 mcg	40
Cerebrolysin	1075 mg	2 mL	537,500 mcg/mL	5,000 mcg	0.9

Reconstitution Guides by Peptide

Expand any peptide below for vial size options, concentration tables, dosing protocol, and storage notes. Each guide ends with a direct link to open that peptide in the calculator.

Also known as: Body Protection Compound 157, PL 14736, Bepecin

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	250 mcg	5
5 mg	2 mL	2,500 mcg/mL	250 mcg	10
5 mg	3 mL	1,667 mcg/mL	250 mcg	15
10 mg	1 mL	10,000 mcg/mL	250 mcg	2.5
10 mg	2 mL	5,000 mcg/mL	250 mcg	5
10 mg	3 mL	3,333 mcg/mL	250 mcg	7.5

Standard dose: 250 mcg

Frequency: Twice daily

Route: Subcutaneous

Timing: Morning and evening, fasted

Half-life: ~4 hours (subcutaneous); shorter intravenous

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open BPC-157 in Calculator →

Also known as: Thymosin Beta-4 fragment, TB4 fragment 17-23, TB500

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	2,500 mcg	50
5 mg	2 mL	2,500 mcg/mL	2,500 mcg	100
5 mg	3 mL	1,667 mcg/mL	2,500 mcg	150
10 mg	1 mL	10,000 mcg/mL	2,500 mcg	25
10 mg	2 mL	5,000 mcg/mL	2,500 mcg	50
10 mg	3 mL	3,333 mcg/mL	2,500 mcg	75

Standard dose: 2,500 mcg

Frequency: Twice weekly

Route: Subcutaneous or intramuscular

Timing: Any time of day

Half-life: ~6 hours subcutaneous; systemic effects last days due to tissue distribution

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open TB-500 in Calculator →

Also known as: NNC 26-0161, Ipamorelin acetate

Available vial sizes: 2 mg, 5 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
2 mg	1 mL	2,000 mcg/mL	200 mcg	10
2 mg	2 mL	1,000 mcg/mL	200 mcg	20
2 mg	3 mL	667 mcg/mL	200 mcg	30
5 mg	1 mL	5,000 mcg/mL	200 mcg	4
5 mg	2 mL	2,500 mcg/mL	200 mcg	8
5 mg	3 mL	1,667 mcg/mL	200 mcg	12

Standard dose: 200 mcg

Frequency: 2-3x daily

Route: Subcutaneous

Timing: Morning fasted, post-workout, and/or before bed

Half-life: ~2 hours (subcutaneous)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Ipamorelin in Calculator →

Also known as: Modified GRF 1-29, Mod GRF(1-29), CJC-1295 without DAC, GRF(1-29)

Available vial sizes: 2 mg, 5 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
2 mg	1 mL	2,000 mcg/mL	100 mcg	5
2 mg	2 mL	1,000 mcg/mL	100 mcg	10
2 mg	3 mL	667 mcg/mL	100 mcg	15
5 mg	1 mL	5,000 mcg/mL	100 mcg	2
5 mg	2 mL	2,500 mcg/mL	100 mcg	4
5 mg	3 mL	1,667 mcg/mL	100 mcg	6

Standard dose: 100 mcg

Frequency: 2-3x daily

Route: Subcutaneous

Timing: Morning fasted, post-workout, before bed - always fasted

Half-life: 30 minutes to 2 hours (subcutaneous); rapid clearance maintains pulsatile pattern

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open CJC-1295 (no DAC) in Calculator →

Also known as: CJC-1295 DAC, DAC:GRF, CJC1295

Available vial sizes: 2 mg, 5 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
2 mg	1 mL	2,000 mcg/mL	2,000 mcg	100
2 mg	2 mL	1,000 mcg/mL	2,000 mcg	200
2 mg	3 mL	667 mcg/mL	2,000 mcg	300
5 mg	1 mL	5,000 mcg/mL	2,000 mcg	40
5 mg	2 mL	2,500 mcg/mL	2,000 mcg	80
5 mg	3 mL	1,667 mcg/mL	2,000 mcg	120

Standard dose: 2,000 mcg

Frequency: Once weekly

Route: Subcutaneous

Timing: Weekly; same day each week

Half-life: ~6-8 days (due to albumin binding via DAC group)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open CJC-1295 (with DAC) in Calculator →

Also known as: AOD9604, Anti-Obesity Drug 9604, HGH Fragment 176-191

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	300 mcg	6
5 mg	2 mL	2,500 mcg/mL	300 mcg	12
5 mg	3 mL	1,667 mcg/mL	300 mcg	18
10 mg	1 mL	10,000 mcg/mL	300 mcg	3
10 mg	2 mL	5,000 mcg/mL	300 mcg	6
10 mg	3 mL	3,333 mcg/mL	300 mcg	9

Standard dose: 300 mcg

Frequency: Once daily

Route: Subcutaneous

Timing: Morning, fasted

Half-life: ~30-60 minutes (subcutaneous)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open AOD-9604 in Calculator →

Also known as: Semax nasal spray, Heptapeptide Semax, MEHFPGP

Available vial sizes: 15 mg, 30 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
15 mg	1 mL	15,000 mcg/mL	300 mcg	2
15 mg	2 mL	7,500 mcg/mL	300 mcg	4
15 mg	3 mL	5,000 mcg/mL	300 mcg	6
30 mg	1 mL	30,000 mcg/mL	300 mcg	1
30 mg	2 mL	15,000 mcg/mL	300 mcg	2
30 mg	3 mL	10,000 mcg/mL	300 mcg	3

Standard dose: 300 mcg

Frequency: Twice daily

Route: Intranasal

Timing: Morning and early afternoon

Half-life: ~Several minutes in plasma; CNS effects persist hours due to BDNF stimulation

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Nasal administration: Use sterile saline (not BAC water) for nasal preparations of this peptide.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Semax in Calculator →

Also known as: TP-7, Selank nasal spray, Threonine-Lysine-Proline-Arg-Pro-Gly-Pro

Available vial sizes: 15 mg, 30 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
15 mg	1 mL	15,000 mcg/mL	250 mcg	1.7
15 mg	2 mL	7,500 mcg/mL	250 mcg	3.3
15 mg	3 mL	5,000 mcg/mL	250 mcg	5
30 mg	1 mL	30,000 mcg/mL	250 mcg	0.8
30 mg	2 mL	15,000 mcg/mL	250 mcg	1.7
30 mg	3 mL	10,000 mcg/mL	250 mcg	2.5

Standard dose: 250 mcg

Frequency: Twice daily

Route: Intranasal

Timing: Morning and evening

Half-life: ~5-10 minutes in plasma; CNS effects persist 4-6 hours

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Nasal administration: Use sterile saline (not BAC water) for nasal preparations of this peptide.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Selank in Calculator →

Also known as: Epitalon, Epithalone, Epithalamin synthetic, AEDG tetrapeptide

Available vial sizes: 10 mg, 50 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
10 mg	1 mL	10,000 mcg/mL	10,000 mcg	100
10 mg	2 mL	5,000 mcg/mL	10,000 mcg	200
10 mg	3 mL	3,333 mcg/mL	10,000 mcg	300
50 mg	1 mL	50,000 mcg/mL	10,000 mcg	20
50 mg	2 mL	25,000 mcg/mL	10,000 mcg	40
50 mg	3 mL	16,667 mcg/mL	10,000 mcg	60

Standard dose: 10,000 mcg

Frequency: Once daily for 10-20 consecutive days

Route: Subcutaneous

Timing: Evening

Half-life: ~30-60 minutes (subcutaneous); epigenetic effects persist long-term

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Epithalon in Calculator →

Also known as: Copper Peptide GHK-Cu, Gly-His-Lys copper complex, Copper Tripeptide-1, GHK copper

Available vial sizes: 50 mg, 200 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
50 mg	1 mL	50,000 mcg/mL	2,000 mcg	4
50 mg	2 mL	25,000 mcg/mL	2,000 mcg	8
50 mg	3 mL	16,667 mcg/mL	2,000 mcg	12
200 mg	1 mL	200,000 mcg/mL	2,000 mcg	1
200 mg	2 mL	100,000 mcg/mL	2,000 mcg	2
200 mg	3 mL	66,667 mcg/mL	2,000 mcg	3

Standard dose: 2,000 mcg

Frequency: Daily or 5x/week

Route: Subcutaneous or topical

Timing: Any time of day

Half-life: ~10-30 minutes in plasma; tissue deposition extends local effects

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open GHK-Cu in Calculator →

Also known as: PT-141, Bremelanotide, Vyleesi, PT141

Available vial sizes: 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
10 mg	1 mL	10,000 mcg/mL	1,000 mcg	10
10 mg	2 mL	5,000 mcg/mL	1,000 mcg	20
10 mg	3 mL	3,333 mcg/mL	1,000 mcg	30

Standard dose: 1,000 mcg

Frequency: As needed

Route: Subcutaneous

Timing: 45-90 minutes before intended sexual activity

Half-life: ~2-3 hours (subcutaneous); onset of effects 30-60 minutes after injection

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open PT-141 / Bremelanotide in Calculator →

Also known as: TH9507, Egrifta, Egrifta SV, Tesamorelin acetate

Available vial sizes: 1 mg, 2 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
1 mg	1 mL	1,000 mcg/mL	2,000 mcg	200
1 mg	2 mL	500 mcg/mL	2,000 mcg	400
1 mg	3 mL	333 mcg/mL	2,000 mcg	600
2 mg	1 mL	2,000 mcg/mL	2,000 mcg	100
2 mg	2 mL	1,000 mcg/mL	2,000 mcg	200
2 mg	3 mL	667 mcg/mL	2,000 mcg	300

Standard dose: 2,000 mcg

Frequency: Once daily

Route: Subcutaneous

Timing: Bedtime, fasted

Half-life: ~30-40 minutes (subcutaneous); daily dosing maintains therapeutic GH pulse

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Tesamorelin in Calculator →

Also known as: DSIP, Delta Sleep Inducing Peptide, Delta sleep peptide

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	200 mcg	4
5 mg	2 mL	2,500 mcg/mL	200 mcg	8
5 mg	3 mL	1,667 mcg/mL	200 mcg	12
10 mg	1 mL	10,000 mcg/mL	200 mcg	2
10 mg	2 mL	5,000 mcg/mL	200 mcg	4
10 mg	3 mL	3,333 mcg/mL	200 mcg	6

Standard dose: 200 mcg

Frequency: Once nightly

Route: Subcutaneous

Timing: 30 minutes before bed

Half-life: ~30 minutes (intravenous); longer with subcutaneous due to slower absorption

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open DSIP / Delta Sleep Inducing Peptide in Calculator →

Also known as: Lys-Pro-Val, alpha-MSH fragment, KPV tripeptide

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	500 mcg	10
5 mg	2 mL	2,500 mcg/mL	500 mcg	20
5 mg	3 mL	1,667 mcg/mL	500 mcg	30
10 mg	1 mL	10,000 mcg/mL	500 mcg	5
10 mg	2 mL	5,000 mcg/mL	500 mcg	10
10 mg	3 mL	3,333 mcg/mL	500 mcg	15

Standard dose: 500 mcg

Frequency: Twice daily

Route: Oral or subcutaneous depending on indication

Timing: With or before meals for gut; any time for subcutaneous

Half-life: ~10-30 minutes (subcutaneous); longer with oral due to local GI action

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open KPV in Calculator →

Also known as: 5-Amino-1-methylquinolinium, 5A1MQ, NNMT inhibitor

Available vial sizes: 100 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
100 mg	1 mL	100,000 mcg/mL	50,000 mcg	50
100 mg	2 mL	50,000 mcg/mL	50,000 mcg	100
100 mg	3 mL	33,333 mcg/mL	50,000 mcg	150

Standard dose: 50,000 mcg

Frequency: Once daily

Route: Oral or subcutaneous

Timing: Morning

Half-life: ~2-4 hours (estimated based on animal pharmacokinetics)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open 5-Amino-1MQ in Calculator →

Also known as: LY3437943, Triple agonist GLP-1/GIP/Glucagon, Eli Lilly LY3437943

Available vial sizes: 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
10 mg	1 mL	10,000 mcg/mL	4,000 mcg	40
10 mg	2 mL	5,000 mcg/mL	4,000 mcg	80
10 mg	3 mL	3,333 mcg/mL	4,000 mcg	120

Standard dose: 4,000 mcg

Frequency: Once weekly

Route: Subcutaneous

Timing: Same day each week

Half-life: ~6-7 days (due to fatty acid acylation enabling albumin binding, similar to semaglutide)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Retatrutide in Calculator →

Also known as: Mounjaro, Zepbound, LY3298176, GLP-1/GIP dual agonist

Available vial sizes: 10 mg, 30 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
10 mg	1 mL	10,000 mcg/mL	5,000 mcg	50
10 mg	2 mL	5,000 mcg/mL	5,000 mcg	100
10 mg	3 mL	3,333 mcg/mL	5,000 mcg	150
30 mg	1 mL	30,000 mcg/mL	5,000 mcg	16.7
30 mg	2 mL	15,000 mcg/mL	5,000 mcg	33.3
30 mg	3 mL	10,000 mcg/mL	5,000 mcg	50

Standard dose: 5,000 mcg

Frequency: Once weekly

Route: Subcutaneous

Timing: Same day each week

Half-life: ~5 days (due to fatty acid-mediated albumin binding, once-weekly dosing)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Tirzepatide in Calculator →

Also known as: Ozempic, Wegovy, Rybelsus, GLP-1 agonist, NNC9204-0092

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	1,000 mcg	20
5 mg	2 mL	2,500 mcg/mL	1,000 mcg	40
5 mg	3 mL	1,667 mcg/mL	1,000 mcg	60
10 mg	1 mL	10,000 mcg/mL	1,000 mcg	10
10 mg	2 mL	5,000 mcg/mL	1,000 mcg	20
10 mg	3 mL	3,333 mcg/mL	1,000 mcg	30

Standard dose: 1,000 mcg

Frequency: Once weekly

Route: Subcutaneous

Timing: Same day each week

Half-life: ~7 days (subcutaneous, due to albumin binding); shorter for oral

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Semaglutide in Calculator →

Also known as: MT-2, Melanotan II, MT2

Available vial sizes: 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
10 mg	1 mL	10,000 mcg/mL	500 mcg	5
10 mg	2 mL	5,000 mcg/mL	500 mcg	10
10 mg	3 mL	3,333 mcg/mL	500 mcg	15

Standard dose: 500 mcg

Frequency: Once daily for 7-10 days loading then 2x/week maintenance

Route: Subcutaneous

Timing: Evening with food to reduce nausea

Half-life: ~30 minutes to 1 hour (subcutaneous); tanning effects persist for weeks after loading

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Melanotan 2 in Calculator →

Also known as: Thymalfasin, Zadaxin, Ta1, TÎ±1

Available vial sizes: 1.6 mg, 5 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
1.6 mg	1 mL	1,600 mcg/mL	1,600 mcg	100
1.6 mg	2 mL	800 mcg/mL	1,600 mcg	200
1.6 mg	3 mL	533 mcg/mL	1,600 mcg	300
5 mg	1 mL	5,000 mcg/mL	1,600 mcg	32
5 mg	2 mL	2,500 mcg/mL	1,600 mcg	64
5 mg	3 mL	1,667 mcg/mL	1,600 mcg	96

Standard dose: 1,600 mcg

Frequency: Twice weekly

Route: Subcutaneous

Timing: Any time

Half-life: ~2 hours (subcutaneous)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Thymosin Alpha-1 in Calculator →

Also known as: Nicotinamide Adenine Dinucleotide, NAD, Beta-NAD+, NAD+ IV

Available vial sizes: 500 mg, 1000 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
500 mg	1 mL	500,000 mcg/mL	500,000 mcg	100
500 mg	2 mL	250,000 mcg/mL	500,000 mcg	200
500 mg	3 mL	166,667 mcg/mL	500,000 mcg	300
1000 mg	1 mL	1,000,000 mcg/mL	500,000 mcg	50
1000 mg	2 mL	500,000 mcg/mL	500,000 mcg	100
1000 mg	3 mL	333,333 mcg/mL	500,000 mcg	150

Standard dose: 500,000 mcg

Frequency: 2-3x weekly subcutaneous or monthly IV drip

Route: Subcutaneous or intravenous

Timing: Morning

Half-life: ~30 minutes (IV); longer subcutaneous absorption provides extended elevation

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Special solvent required: bacteriostatic saline

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open NAD+ in Calculator →

Also known as: Growth Hormone Releasing Peptide 2, KP-102, Pralmorelin

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	100 mcg	2
5 mg	2 mL	2,500 mcg/mL	100 mcg	4
5 mg	3 mL	1,667 mcg/mL	100 mcg	6
10 mg	1 mL	10,000 mcg/mL	100 mcg	1
10 mg	2 mL	5,000 mcg/mL	100 mcg	2
10 mg	3 mL	3,333 mcg/mL	100 mcg	3

Standard dose: 100 mcg

Frequency: 2-3x daily

Route: Subcutaneous

Timing: Morning fasted, post-workout, before bed

Half-life: ~15-30 minutes (subcutaneous)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open GHRP-2 in Calculator →

Also known as: Growth Hormone Releasing Peptide 6, His-D-Trp-Ala-Trp-D-Phe-Lys hexapeptide

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	100 mcg	2
5 mg	2 mL	2,500 mcg/mL	100 mcg	4
5 mg	3 mL	1,667 mcg/mL	100 mcg	6
10 mg	1 mL	10,000 mcg/mL	100 mcg	1
10 mg	2 mL	5,000 mcg/mL	100 mcg	2
10 mg	3 mL	3,333 mcg/mL	100 mcg	3

Standard dose: 100 mcg

Frequency: 2-3x daily

Route: Subcutaneous

Timing: Morning and/or before bed, post-workout

Half-life: ~15-20 minutes (subcutaneous)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open GHRP-6 in Calculator →

Also known as: Examorelin, EP-23905, MF-6003, His-D-2MeTrp-Ala-Trp-D-Phe-Lys hexapeptide

Available vial sizes: 2 mg, 5 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
2 mg	1 mL	2,000 mcg/mL	200 mcg	10
2 mg	2 mL	1,000 mcg/mL	200 mcg	20
2 mg	3 mL	667 mcg/mL	200 mcg	30
5 mg	1 mL	5,000 mcg/mL	200 mcg	4
5 mg	2 mL	2,500 mcg/mL	200 mcg	8
5 mg	3 mL	1,667 mcg/mL	200 mcg	12

Standard dose: 200 mcg

Frequency: Twice daily

Route: Subcutaneous

Timing: Morning fasted and before bed

Half-life: ~30 minutes (subcutaneous)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Hexarelin in Calculator →

Also known as: Ibutamoren, MK677, L-163,191, Nutrabol

Available vial sizes: 25 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
25 mg	1 mL	25,000 mcg/mL	15,000 mcg	60
25 mg	2 mL	12,500 mcg/mL	15,000 mcg	120
25 mg	3 mL	8,333 mcg/mL	15,000 mcg	180

Standard dose: 15,000 mcg

Frequency: Once daily oral

Route: Oral

Timing: Before bed

Half-life: ~24 hours (oral) - unique among GH secretagogues for once-daily oral dosing

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open MK-677 in Calculator →

Also known as: GHRH(1-29)-NH2, GRF(1-29), Sermorelin acetate, Geref

Available vial sizes: 3 mg, 9 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
3 mg	1 mL	3,000 mcg/mL	300 mcg	10
3 mg	2 mL	1,500 mcg/mL	300 mcg	20
3 mg	3 mL	1,000 mcg/mL	300 mcg	30
9 mg	1 mL	9,000 mcg/mL	300 mcg	3.3
9 mg	2 mL	4,500 mcg/mL	300 mcg	6.7
9 mg	3 mL	3,000 mcg/mL	300 mcg	10

Standard dose: 300 mcg

Frequency: Once daily or twice daily

Route: Subcutaneous

Timing: Before bed; second dose morning fasted if twice daily

Half-life: ~10-20 minutes (intravenous); ~30-60 minutes subcutaneous

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Sermorelin in Calculator →

Also known as: Long Arg3 IGF-1, Insulin-like Growth Factor 1 LR3, LR3-IGF-1

Available vial sizes: 1 mg, 0.1 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
1 mg	1 mL	1,000 mcg/mL	40 mcg	4
1 mg	2 mL	500 mcg/mL	40 mcg	8
1 mg	3 mL	333 mcg/mL	40 mcg	12
0.1 mg	1 mL	100 mcg/mL	40 mcg	40
0.1 mg	2 mL	50 mcg/mL	40 mcg	80
0.1 mg	3 mL	33 mcg/mL	40 mcg	120

Standard dose: 40 mcg

Frequency: Daily for 4 weeks

Route: Subcutaneous or intramuscular (near muscles worked)

Timing: Post-workout; 50% systemic, 50% near target muscles for local hypertrophy

Half-life: ~20 hours (subcutaneous; dramatically extended vs native IGF-1 due to reduced IGFBP binding)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Special solvent required: 0.6% acetic acid

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open IGF-1 LR3 in Calculator →

Also known as: PEGylated Mechano Growth Factor, Pegylated MGF, PEG-IGF-1Ec

Available vial sizes: 2 mg, 5 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
2 mg	1 mL	2,000 mcg/mL	300 mcg	15
2 mg	2 mL	1,000 mcg/mL	300 mcg	30
2 mg	3 mL	667 mcg/mL	300 mcg	45
5 mg	1 mL	5,000 mcg/mL	300 mcg	6
5 mg	2 mL	2,500 mcg/mL	300 mcg	12
5 mg	3 mL	1,667 mcg/mL	300 mcg	18

Standard dose: 300 mcg

Frequency: Once daily for 4-6 weeks

Route: Intramuscular near trained muscles

Timing: Post-workout; inject near muscles trained that day

Half-life: ~24 hours (due to PEGylation); native MGF: ~5 minutes

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open PEG-MGF in Calculator →

Also known as: FST-344, Follistatin, FS-344

Available vial sizes: 1 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
1 mg	1 mL	1,000 mcg/mL	100 mcg	10
1 mg	2 mL	500 mcg/mL	100 mcg	20
1 mg	3 mL	333 mcg/mL	100 mcg	30

Standard dose: 100 mcg

Frequency: Daily for 10 days

Route: Intramuscular near target muscle groups

Timing: Post-workout

Half-life: ~1-2 hours (subcutaneous); biological effects on muscle last days due to receptor neutralization

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Follistatin 344 in Calculator →

Also known as: Love hormone, Pitocin, Syntocinon, CYIQNCPLG

Available vial sizes: 2 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
2 mg	1 mL	2,000 mcg/mL	20 mcg	1
2 mg	2 mL	1,000 mcg/mL	20 mcg	2
2 mg	3 mL	667 mcg/mL	20 mcg	3

Standard dose: 20 mcg

Frequency: As needed, maximum 1-2x daily

Route: Intranasal

Timing: 20-30 minutes before social or intimate activity

Half-life: ~1-6 minutes (plasma half-life very short); intranasal effects persist 45-90 minutes

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Nasal administration: Use sterile saline (not BAC water) for nasal preparations of this peptide.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Oxytocin in Calculator →

Also known as: Cathelicidin LL-37, hCAP18/LL-37, Human cathelicidin antimicrobial peptide

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	200 mcg	4
5 mg	2 mL	2,500 mcg/mL	200 mcg	8
5 mg	3 mL	1,667 mcg/mL	200 mcg	12
10 mg	1 mL	10,000 mcg/mL	200 mcg	2
10 mg	2 mL	5,000 mcg/mL	200 mcg	4
10 mg	3 mL	3,333 mcg/mL	200 mcg	6

Standard dose: 200 mcg

Frequency: Daily for wound healing; 2-3x daily for active infections

Route: Subcutaneous near target tissue or topical

Timing: Any time; consistent schedule

Half-life: ~10-30 minutes (plasma; rapidly degraded by proteases; local effects at application site persist longer)

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open LL-37 in Calculator →

Also known as: PNB-0408, N-hexanoic-Tyr-Ile-(6) aminohexanoic amide

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	1,000 mcg	20
5 mg	2 mL	2,500 mcg/mL	1,000 mcg	40
5 mg	3 mL	1,667 mcg/mL	1,000 mcg	60
10 mg	1 mL	10,000 mcg/mL	1,000 mcg	10
10 mg	2 mL	5,000 mcg/mL	1,000 mcg	20
10 mg	3 mL	3,333 mcg/mL	1,000 mcg	30

Standard dose: 1,000 mcg

Frequency: 3–5x per week

Route: Subcutaneous

Timing: Morning

Half-life: Estimated several hours; brain retention prolonged due to lipophilicity

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Dihexa in Calculator →

Also known as: Elamipretide, MTP-131, Bendavia, Szeto-Schiller peptide 31

Available vial sizes: 50 mg, 100 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
50 mg	1 mL	50,000 mcg/mL	10,000 mcg	20
50 mg	2 mL	25,000 mcg/mL	10,000 mcg	40
50 mg	3 mL	16,667 mcg/mL	10,000 mcg	60
100 mg	1 mL	100,000 mcg/mL	10,000 mcg	10
100 mg	2 mL	50,000 mcg/mL	10,000 mcg	20
100 mg	3 mL	33,333 mcg/mL	10,000 mcg	30

Standard dose: 10,000 mcg

Frequency: Daily or 5x per week

Route: Subcutaneous

Timing: Morning or pre-exercise

Half-life: ~2–3 hours plasma half-life; mitochondrial accumulation persists longer

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open SS-31 in Calculator →

Also known as: HN, Humanin peptide, MT-RNR2 derived peptide

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	1,000 mcg	20
5 mg	2 mL	2,500 mcg/mL	1,000 mcg	40
5 mg	3 mL	1,667 mcg/mL	1,000 mcg	60
10 mg	1 mL	10,000 mcg/mL	1,000 mcg	10
10 mg	2 mL	5,000 mcg/mL	1,000 mcg	20
10 mg	3 mL	3,333 mcg/mL	1,000 mcg	30

Standard dose: 1,000 mcg

Frequency: 5x per week

Route: Subcutaneous

Timing: Morning

Half-life: ~5–15 minutes (native); analogs like HNG have longer half-lives

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Humanin in Calculator →

Also known as: Mitochondrial ORF of the 12S rRNA type-c, MT-ORF peptide

Available vial sizes: 10 mg, 25 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
10 mg	1 mL	10,000 mcg/mL	5,000 mcg	50
10 mg	2 mL	5,000 mcg/mL	5,000 mcg	100
10 mg	3 mL	3,333 mcg/mL	5,000 mcg	150
25 mg	1 mL	25,000 mcg/mL	5,000 mcg	20
25 mg	2 mL	12,500 mcg/mL	5,000 mcg	40
25 mg	3 mL	8,333 mcg/mL	5,000 mcg	60

Standard dose: 5,000 mcg

Frequency: 5x per week

Route: Subcutaneous

Timing: Pre-exercise or morning fasted

Half-life: ~2–5 hours plasma half-life

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open MOTS-c in Calculator →

Also known as: GnRH, Gonadotropin-releasing hormone, Luteinizing hormone-releasing hormone, LHRH, Factrel

Available vial sizes: 2 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
2 mg	1 mL	2,000 mcg/mL	100 mcg	5
2 mg	2 mL	1,000 mcg/mL	100 mcg	10
2 mg	3 mL	667 mcg/mL	100 mcg	15
10 mg	1 mL	10,000 mcg/mL	100 mcg	1
10 mg	2 mL	5,000 mcg/mL	100 mcg	2
10 mg	3 mL	3,333 mcg/mL	100 mcg	3

Standard dose: 100 mcg

Frequency: Twice daily

Route: Subcutaneous

Timing: Morning and evening

Half-life: ~2–10 minutes (endogenous); slightly longer for synthetic Gonadorelin acetate

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Gonadorelin in Calculator →

Also known as: KP-10, Metastin (45-54), KISS1 peptide, Kisspeptin 10

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	100 mcg	2
5 mg	2 mL	2,500 mcg/mL	100 mcg	4
5 mg	3 mL	1,667 mcg/mL	100 mcg	6
10 mg	1 mL	10,000 mcg/mL	100 mcg	1
10 mg	2 mL	5,000 mcg/mL	100 mcg	2
10 mg	3 mL	3,333 mcg/mL	100 mcg	3

Standard dose: 100 mcg

Frequency: Twice to three times daily

Route: Subcutaneous

Timing: Morning, midday, and evening

Half-life: ~10–27 minutes (native Kisspeptin-10); very short, requiring frequent dosing

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Kisspeptin-10 in Calculator →

Also known as: Melanotan I, Afamelanotide, CUV1647, Scenesse, NDP-alpha-MSH

Available vial sizes: 10 mg, 20 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
10 mg	1 mL	10,000 mcg/mL	1,000 mcg	10
10 mg	2 mL	5,000 mcg/mL	1,000 mcg	20
10 mg	3 mL	3,333 mcg/mL	1,000 mcg	30
20 mg	1 mL	20,000 mcg/mL	1,000 mcg	5
20 mg	2 mL	10,000 mcg/mL	1,000 mcg	10
20 mg	3 mL	6,667 mcg/mL	1,000 mcg	15

Standard dose: 1,000 mcg

Frequency: Daily during loading; 2–3x per week maintenance

Route: Subcutaneous

Timing: Evening

Half-life: ~15–17 hours; much longer than native alpha-MSH due to analog stability

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open MT-1 (Melanotan 1) in Calculator →

Also known as: Vasoactive Intestinal Peptide, VIP peptide, PHI-27

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	100 mcg	2
5 mg	2 mL	2,500 mcg/mL	100 mcg	4
5 mg	3 mL	1,667 mcg/mL	100 mcg	6
10 mg	1 mL	10,000 mcg/mL	100 mcg	1
10 mg	2 mL	5,000 mcg/mL	100 mcg	2
10 mg	3 mL	3,333 mcg/mL	100 mcg	3

Standard dose: 100 mcg

Frequency: Twice daily

Route: Intranasal or subcutaneous

Timing: Morning and evening

Half-life: ~2 minutes in plasma (rapidly degraded by plasma peptidases); intranasal delivery extends CNS exposure

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Nasal administration: Use sterile saline (not BAC water) for nasal preparations of this peptide.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open VIP (Vasoactive Intestinal Peptide) in Calculator →

Also known as: SNAP-8, Acetyl Glutamyl Heptapeptide-3, Leuphasyl-Argireline, Syn-Ake alternative

Available vial sizes: 100 mg

Reconstitution reference

Vial size	BAC water	Concentration
100 mg	1 mL	100,000 mcg/mL
100 mg	2 mL	50,000 mcg/mL
100 mg	3 mL	33,333 mcg/mL

Standard dose: 0 mcg

Frequency: Twice daily (morning and evening)

Route: Topical

Timing: Morning and evening skincare routine

Half-life: Topical application — not applicable in systemic terms; local residence in skin

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Snap-8 in Calculator →

Also known as: Acetyl Hexapeptide-3, Acetyl Hexapeptide-8, Leuphasyl synergist, AH3

Available vial sizes: 100 mg

Reconstitution reference

Vial size	BAC water	Concentration
100 mg	1 mL	100,000 mcg/mL
100 mg	2 mL	50,000 mcg/mL
100 mg	3 mL	33,333 mcg/mL

Standard dose: 0 mcg

Frequency: Twice daily

Route: Topical

Timing: Morning and evening

Half-life: Topical — not systemically absorbed in meaningful amounts

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Argireline in Calculator →

Also known as: Acetyl Tyr-D-Ala-Gly-Phe-Leu-OH, Enkephalin analog, Argireline synergist

Available vial sizes: 100 mg

Reconstitution reference

Vial size	BAC water	Concentration
100 mg	1 mL	100,000 mcg/mL
100 mg	2 mL	50,000 mcg/mL
100 mg	3 mL	33,333 mcg/mL

Standard dose: 0 mcg

Frequency: Twice daily

Route: Topical

Timing: Morning and evening

Half-life: Topical application — not systemically relevant

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Leuphasyl in Calculator →

Also known as: Pal-GHK, Palmitoyl-Gly-His-Lys, Matrixyl 3000 component, Biopeptide EL

Available vial sizes: 100 mg

Reconstitution reference

Vial size	BAC water	Concentration
100 mg	1 mL	100,000 mcg/mL
100 mg	2 mL	50,000 mcg/mL
100 mg	3 mL	33,333 mcg/mL

Standard dose: 0 mcg

Frequency: Twice daily

Route: Topical

Timing: Morning and evening after cleansing

Half-life: Topical — dermally retained; systemic absorption minimal

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Palmitoyl Tripeptide-1 in Calculator →

Also known as: GHK tripeptide, Glycyl-L-histidyl-L-lysine, Wound healing tripeptide

Available vial sizes: 50 mg, 100 mg

Reconstitution reference

Vial size	BAC water	Concentration
50 mg	1 mL	50,000 mcg/mL
50 mg	2 mL	25,000 mcg/mL
50 mg	3 mL	16,667 mcg/mL
100 mg	1 mL	100,000 mcg/mL
100 mg	2 mL	50,000 mcg/mL
100 mg	3 mL	33,333 mcg/mL

Standard dose: 0 mcg

Frequency: Twice daily topical / daily subcutaneous

Route: Topical or subcutaneous

Timing: Morning and evening

Half-life: Rapid plasma clearance (minutes); topically, skin residence time is extended

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open GHK (Gly-His-Lys) in Calculator →

Also known as: GHK-Cu topical 3%, 3% copper peptide serum, High-strength copper peptide

Available vial sizes: 500 mg

Reconstitution reference

Vial size	BAC water	Concentration
500 mg	1 mL	500,000 mcg/mL
500 mg	2 mL	250,000 mcg/mL
500 mg	3 mL	166,667 mcg/mL

Standard dose: 0 mcg

Frequency: Once daily (evening)

Route: Topical

Timing: Evening after cleansing and toning

Half-life: Topical skin residence; not systemically absorbed in meaningful amounts at topical doses

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Copper Peptide GHK-Cu 3% in Calculator →

Also known as: Thymosin Beta-4 Fragment, Ac-SDKP, N-acetyl-seryl-aspartyl-lysyl-proline, TB4 Fragment 17-23

Available vial sizes: 5 mg, 10 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
5 mg	1 mL	5,000 mcg/mL	1,000 mcg	20
5 mg	2 mL	2,500 mcg/mL	1,000 mcg	40
5 mg	3 mL	1,667 mcg/mL	1,000 mcg	60
10 mg	1 mL	10,000 mcg/mL	1,000 mcg	10
10 mg	2 mL	5,000 mcg/mL	1,000 mcg	20
10 mg	3 mL	3,333 mcg/mL	1,000 mcg	30

Standard dose: 1,000 mcg

Frequency: 5x per week

Route: Subcutaneous

Timing: Morning

Half-life: ~17 minutes endogenous plasma half-life (ACE cleaves it); exogenous TB4-Frag has similar short half-life

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open TB4-Frag in Calculator →

Also known as: FPF 1070, EVER Neuro Pharma peptides, Porcine brain peptide mixture

Available vial sizes: 1075 mg

Reconstitution reference

Vial size	BAC water	Concentration	Std dose	U-100 units
1075 mg	1 mL	1,075,000 mcg/mL	5,000 mcg	0.5
1075 mg	2 mL	537,500 mcg/mL	5,000 mcg	0.9
1075 mg	3 mL	358,333 mcg/mL	5,000 mcg	1.4

Standard dose: 5,000 mcg

Frequency: Daily for 20 days (clinical protocol)

Route: IV infusion or IM

Timing: Morning, slow IV infusion or deep IM

Half-life: Variable — mixture of peptides; peptide fractions have short plasma half-lives but CNS effects persist

Research stage: Preclinical

Reconstitution: Add bacteriostatic water slowly down the side of the vial. Swirl gently; do not shake. Solution should be clear and colourless.

Storage (lyophilised): Store lyophilized at −20°C for up to 2 years. Stable at room temperature (below 25°C) for up to 3 months if kept dry and away from light.

Storage (reconstituted): Refrigerate at 2–8°C. Use within 28–30 days of reconstitution. Do not freeze the reconstituted solution.

Open Cerebrolysin in Calculator →

How to Reconstitute Peptides: Step-by-Step Guide

Supplies needed

Lyophilised peptide vial
Bacteriostatic water (BAC water) — 30mL multi-dose vial
U-100 insulin syringes (for reconstitution and dosing)
Alcohol swabs (70% isopropyl)
Sharps disposal container
Permanent marker (for labelling)
Refrigerator

Gather and inspect

Check both vials for cracks, cloudiness in the BAC water, or discolouration. The peptide powder should be white or off-white. Wash hands thoroughly and work on a clean surface.

Swab the tops

Wipe the rubber septum of both the peptide vial and BAC water vial with a fresh alcohol swab. Allow to air-dry for 10–15 seconds before piercing — do not blow or fan dry.

Draw bacteriostatic water

Using a fresh syringe, draw the calculated volume of BAC water from the BAC water vial. For most peptides, 1–2mL is standard. Use the calculator above to determine the exact volume.

Inject down the vial wall at 45°

Tilt the peptide vial at 45 degrees. Slowly depress the plunger so the water runs down the inside wall of the vial, not directly onto the powder cake. This prevents denaturation from mechanical force.

Swirl — do not shake

Gently roll the vial between your palms in a slow circular motion until the powder fully dissolves. The solution should become clear and colourless. Never shake — agitation denatures peptide bonds.

Label and refrigerate

Write the peptide name, date reconstituted, concentration (mcg/mL), and vial size on the label using a permanent marker. Store immediately at 2–8°C, away from the refrigerator door. Discard after 28–30 days.

How to Read an Insulin Syringe for Peptide Dosing

Syringe units are a volume measurement only — they are not International Units (IU) of any drug. On a U-100 syringe, one unit = 0.01mL. The marking system exists purely to measure volume; the term “unit” here has no relationship to hormonal or biological units of activity.

Syringe comparison: 0.3mL vs 0.5mL vs 1.0mL

U-100 0.3mL

30 total units • 1 unit increments • Best for doses under 20 units • Most precise

U-100 0.5mL

50 total units • 1 unit increments • Good for doses 10–40 units • Most common

U-100 1.0mL

100 total units • 2 unit increments • For larger volumes • Use for GLP-1 doses

mcg per unit — conversion reference

Concentration	1 unit =	5 units =	10 units =	20 units =	50 units =
500 mcg/mL	5 mcg	25 mcg	50 mcg	100 mcg	250 mcg
1,000 mcg/mL	10 mcg	50 mcg	100 mcg	200 mcg	500 mcg
2,500 mcg/mL	25 mcg	125 mcg	250 mcg	500 mcg	1250 mcg
5,000 mcg/mL	50 mcg	250 mcg	500 mcg	1000 mcg	2500 mcg
10,000 mcg/mL	100 mcg	500 mcg	1000 mcg	2000 mcg	5000 mcg

Bacteriostatic Water vs Sterile Water for Peptides: Which Should You Use?

	Bacteriostatic Water	Sterile Water	Normal Saline (0.9%)	BAC Saline
Preservative	0.9% benzyl alcohol	None	None	0.9% benzyl alcohol
Shelf life (opened)	28 days refrigerated	Single use only	Single use only	28 days refrigerated
Multi-dose safe	Yes	No	No	Yes
Best for	Most research peptides	One-off single doses	Nasal sprays	NAD+, nasal multi-dose

NAD+ requires BAC saline

NAD+ (nicotinamide adenine dinucleotide) is best reconstituted with bacteriostatic saline (0.9% NaCl with 0.9% benzyl alcohol) rather than standard BAC water. This improves stability and is isotonic, which reduces injection discomfort.

Nasal peptides need sterile saline

Peptides administered intranasally — including Semax, Selank, VIP, and Oxytocin — should be reconstituted in sterile saline (0.9% NaCl, no preservative) or sterile water. Benzyl alcohol is a mucosal irritant at nasal concentrations and should be avoided for intranasal use.

How Long Do Reconstituted Peptides Last? Storage Guide

Storage condition	Duration	Notes
Lyophilised, −20°C freezer	Up to 2 years	Optimal long-term storage
Lyophilised, room temp (<25°C, dry)	2–3 months	Keep sealed and away from light
Reconstituted, 2–8°C refrigerator	28–30 days	Most common research use case
Reconstituted, room temperature	<24 hours	Not recommended for repeated use
Reconstituted, frozen at −20°C	Not recommended	Freeze-thaw cycles degrade peptides

Always label the vial with the reconstitution date and discard after 28 days regardless of how much remains.
Store reconstituted vials upright in the main body of the refrigerator, not in the door (temperature fluctuates there).
Protect from light — a small piece of aluminium foil around the vial prevents photodegradation.
Never freeze a reconstituted solution; ice crystal formation during freezing mechanically damages peptide structure.
Keep lyophilised vials away from humidity — use desiccant packs in storage containers.
If the solution appears cloudy, discoloured, or has visible particulates after reconstitution, discard it.

10 Common Peptide Reconstitution Mistakes to Avoid

Confusing mg and mcg. One milligram equals 1,000 micrograms. A 5mg vial contains 5,000mcg. Always convert to the same unit before calculating. The calculator handles this automatically.
Confusing syringe units with International Units. On an insulin syringe, “units” measure volume only. 10 units on a U-100 syringe = 0.1mL. This has nothing to do with IU of hormones or other biologics.
Shaking the vial. Vigorous shaking creates foam and mechanical stress that denatures peptide bonds. Always swirl gently in a circular motion until the powder dissolves completely.
Spraying water directly onto the powder. Direct water pressure disrupts the lyophilised cake. Instead, tilt the vial and let BAC water run slowly down the inner wall to dissolve the powder from the sides.
Using sterile water for multi-dose vials. Sterile water contains no preservative. Using it for repeated-access vials creates bacterial contamination risk. Use bacteriostatic water for any vial you will access more than once.
Adding the wrong BAC water volume. Changing the BAC water volume changes the concentration and therefore the units per dose. Always re-calculate units if you use a different water volume than planned.
Failing to label the vial. An unlabelled vial is a safety hazard. Label with: peptide name, date mixed, concentration (mcg/mL), and your initials. Discard any unlabelled reconstituted vials.
Storing in the refrigerator door. Door temperatures fluctuate every time the fridge opens. Store peptide vials in the main body of the refrigerator where temperature remains stable at 2–8°C.
Using a vial beyond 30 days. Even if solution remains in the vial, most reconstituted peptides degrade meaningfully after 28–30 days. Discard on or before the 28-day mark from the reconstitution date.
Not swabbing the septum before drawing. Always swab the rubber septum with a fresh alcohol swab before each draw, even if the vial was recently opened. Allow to air-dry before piercing.

Frequently Asked Questions About Peptide Reconstitution

Browse Peptide Protocols and Dosing Guides

Each peptide page includes a full protocol, dosing table, half-life, storage guide, and direct link to the reconstitution calculator.

5-Amino-1MQ AOD-9604 Argireline BPC-157 Cerebrolysin CJC-1295 (no DAC) CJC-1295 (with DAC) Copper Peptide GHK-Cu 3% Dihexa DSIP / Delta Sleep Inducing Peptide Epithalon Follistatin 344 GHK (Gly-His-Lys) GHK-Cu GHRP-2 GHRP-6 Gonadorelin Hexarelin Humanin IGF-1 LR3 Ipamorelin Kisspeptin-10 KPV Leuphasyl LL-37 Melanotan 2 MK-677 MOTS-c MT-1 (Melanotan 1) NAD+ Oxytocin Palmitoyl Tripeptide-1 PEG-MGF PT-141 / Bremelanotide Retatrutide Selank Semaglutide Semax Sermorelin Snap-8 SS-31 TB-500 TB4-Frag Tesamorelin Thymosin Alpha-1 Tirzepatide VIP (Vasoactive Intestinal Peptide)

Research Purposes Only — Not Medical Advice

All content on this page, including the calculator, charts, and guides, is provided for educational and research purposes only. Peptides described on this site are research chemicals not approved by the FDA or equivalent regulatory bodies for human consumption. This content does not constitute medical advice, diagnosis, or treatment. Always consult a qualified and licensed medical professional before using any compound. The calculator results are mathematical computations based on user inputs and carry no warranty of accuracy for any specific application.

Tirzepatide Dosage & Reconstitution Calculator

Supplies needed per session

How to Calculate Peptide Reconstitution in 3 Steps

Calculate concentration

Calculate injection volume

Convert to syringe units

What is concentration and why does it matter?

Peptide Reconstitution Chart: Pre-Calculated Common Doses

Reconstitution Guides by Peptide

BPC-157 Dosage Calculator

TB-500 Dosage Calculator

Ipamorelin Dosage Calculator

CJC-1295 Ipamorelin Dosage Calculator

CJC-1295 DAC Dosage Calculator

AOD-9604 Dosage Calculator

Semax Reconstitution Guide

Selank Reconstitution Guide

Epithalon Dosage Calculator

GHK-Cu Dosage Calculator

PT-141 Dosage Calculator

Tesamorelin Dosage Calculator

DSIP Dosage Calculator

KPV Peptide Dosage Calculator

5-Amino-1MQ Dosage Calculator

Retatrutide Reconstitution Calculator

Tirzepatide Reconstitution Calculator

Semaglutide Reconstitution Calculator

Melanotan 2 Dosage Calculator

Thymosin Alpha-1 Dosage Calculator

NAD+ Reconstitution Guide

GHRP-2 Dosage Calculator

GHRP-6 Dosage Calculator

Hexarelin Dosage Calculator

MK-677 Dosage Calculator

Sermorelin Dosage Calculator

IGF-1 LR3 Dosage Calculator

PEG-MGF Dosage Calculator

Follistatin 344 Dosage Calculator

Oxytocin Peptide Dosage Calculator

LL-37 Peptide Dosage Calculator

Dihexa Dosage Calculator

SS-31 Elamipretide Dosage Calculator

Humanin Peptide Dosage Calculator

MOTS-c Dosage Calculator

Gonadorelin Dosage Calculator

Kisspeptin-10 Dosage Calculator

Melanotan 1 Dosage Calculator

VIP Peptide Dosage Calculator

Snap-8 Dosage Calculator

Argireline Dosage Calculator

Leuphasyl Dosage Calculator

Palmitoyl Tripeptide-1 Dosage Calculator

GHK (Gly-His-Lys) Dosage Calculator

Copper Peptide GHK-Cu 3% Dosage Calculator

TB4-Frag Dosage Calculator

Cerebrolysin Dosage Calculator

How to Reconstitute Peptides: Step-by-Step Guide

Supplies needed

Gather and inspect

Swab the tops

Draw bacteriostatic water

Inject down the vial wall at 45°

Swirl — do not shake

Label and refrigerate

How to Read an Insulin Syringe for Peptide Dosing

Syringe comparison: 0.3mL vs 0.5mL vs 1.0mL

mcg per unit — conversion reference

Bacteriostatic Water vs Sterile Water for Peptides: Which Should You Use?

NAD+ requires BAC saline

Nasal peptides need sterile saline

How Long Do Reconstituted Peptides Last? Storage Guide

10 Common Peptide Reconstitution Mistakes to Avoid

Frequently Asked Questions About Peptide Reconstitution

How do you calculate peptide reconstitution?

How many mL of bacteriostatic water do I add to a peptide vial?

How do I know how much water to put in my peptide?

How long do reconstituted peptides last?

What is the difference between bacteriostatic water and sterile water for peptides?

How do I read an insulin syringe for peptide dosing?

What does it mean to reconstitute a peptide?