5-Amino-1MQ
Also known as: 5-Amino-1-methylquinolinium · 5A1MQ · NNMT inhibitor
5-Amino-1MQ inhibits NNMT (Nicotinamide N-methyltransferase), an enzyme that catabolizes nicotinamide (NAD+ precursor) by methylating it to 1-methylnicotinamide. By inhibiting NNMT, 5-Amino-1MQ increases available nicotinamide for NAD+ synthesis, elevating intracellular NAD+ levels. This activates sirtuin enzymes (SIRT1, SIRT3) and AMPK, shifting...
What It Is
5-Amino-1MQ inhibits NNMT (Nicotinamide N-methyltransferase), an enzyme that catabolizes nicotinamide (NAD+ precursor) by methylating it to 1-methylnicotinamide. By inhibiting NNMT, 5-Amino-1MQ increases available nicotinamide for NAD+ synthesis, elevating intracellular NAD+ levels. This activates sirtuin enzymes (SIRT1, SIRT3) and AMPK, shifting cellular metabolism toward fat oxidation and energy expenditure. In adipocytes, NNMT inhibition reduces fat cell size and lipogenesis gene expression while increasing lipolysis. Animal studies show significant body fat reduction without caloric restriction.
Supplies Needed
Reconstitution solvent for injectable use
Subcutaneous injection
Sterilization
Storage
Store at -20C. Stable at room temperature for several weeks in powder form.
Refrigerate at 2-8C; use within 30 days.
This information is provided for educational and research purposes only. Not approved for human consumption by the FDA or any regulatory body. Always consult a qualified medical professional.
Dosing Protocols
Timing: Morning with or without food. Start with oral if available; subcutaneous injection for research doses
↓ Apply these values to the reconstitution calculatorTiming: Morning. 100 mg daily is the most common research dose for body composition; combine with caloric deficit
↓ Apply these values to the reconstitution calculatorTiming: Morning and noon. Twice-daily dosing for sustained NNMT inhibition; stack with NAD+ precursors like NMN for additive NAD+ elevation
↓ Apply these values to the reconstitution calculatorWeekly Timeline
| Week | Expected Effects |
|---|---|
| Week 1 | Elevated NAD+ levels; possible mild increase in energy and metabolic rate |
| Week 2 | Early fat mobilization; improved energy metabolism |
| Week 4 | Noticeable fat loss, particularly visceral fat |
| Week 8 | Significant body composition improvement; sustained fat loss |
Reconstitution Calculator
1 unit on U-100 syringe = 0.01 mL · Always label your vial after reconstitution
Injection Technique & Reconstitution
- Remove flip-top cap from the vial
- Swab rubber stopper with alcohol swab
- Draw 2 mL bacteriostatic water into syringe
- Inject slowly down the vial wall
- Gently swirl until dissolved
- Label with date; refrigerate at 2-8C
Use our free peptide reconstitution calculator to get the exact syringe units for your vial size and dose.
Mechanism of Action
5-Amino-1MQ inhibits NNMT (Nicotinamide N-methyltransferase), an enzyme that catabolizes nicotinamide (NAD+ precursor) by methylating it to 1-methylnicotinamide. By inhibiting NNMT, 5-Amino-1MQ increases available nicotinamide for NAD+ synthesis, elevating intracellular NAD+ levels. This activates sirtuin enzymes (SIRT1, SIRT3) and AMPK, shifting cellular metabolism toward fat oxidation and energy expenditure. In adipocytes, NNMT inhibition reduces fat cell size and lipogenesis gene expression while increasing lipolysis. Animal studies show significant body fat reduction without caloric restriction.
Key Research Papers
Demonstrated that 5-Amino-1MQ treatment in diet-induced obese mice produced significant reduction in body fat and adipocyte size without changes in food intake.
View on PubMed →Stacks Well With
Complementary fat loss mechanisms - AOD-9604 activates beta-adrenergic lipolysis while 5-Amino-1MQ inhibits NNMT and elevates NAD+ for metabolic fat burning.
Triple fat loss approach: GLP-1 receptor agonist for appetite suppression (Semaglutide), NNMT inhibition for metabolic fat burning (5-Amino-1MQ), and a caloric deficit for comprehensive body composition improvement.
Frequently Asked Questions
5-Amino-1MQ is a small molecule NNMT inhibitor that increases cellular NAD+ levels by blocking the enzyme that degrades nicotinamide. This results in fat loss, improved metabolism, and potential anti-aging effects via sirtuin activation.
No - it is a small molecule quinolinium compound, not a peptide. However, it is commonly grouped with research peptides in the performance and anti-aging space due to similar research and vendor distribution channels.
NMN and NR are NAD+ precursors that increase NAD+ by providing substrate. 5-Amino-1MQ increases NAD+ by inhibiting its degradation. They work via different pathways and can be stacked for additive NAD+ elevation.
Legal Status by Region
This information is provided for educational and research purposes only. Not approved for human consumption by the FDA or any regulatory body. Always consult a qualified medical professional.