Kisspeptin-10
Also known as: KP-10 · Metastin (45-54) · KISS1 peptide · Kisspeptin 10
Kisspeptin-10 (the C-terminal decapeptide of kisspeptin-54) binds to GPR54 (KISS1R) receptors on hypothalamic GnRH neurons with high affinity. This triggers Gq/11 signaling, phospholipase C activation, IP3-mediated calcium release, and ultimately stimulates a burst of GnRH secretion. The GnRH pulse then...
What It Is
Kisspeptin-10 (the C-terminal decapeptide of kisspeptin-54) binds to GPR54 (KISS1R) receptors on hypothalamic GnRH neurons with high affinity. This triggers Gq/11 signaling, phospholipase C activation, IP3-mediated calcium release, and ultimately stimulates a burst of GnRH secretion. The GnRH pulse then triggers LH and FSH release from the pituitary. Kisspeptin neurons in the hypothalamus (particularly in the arcuate nucleus and anteroventral periventricular nucleus) are the master regulators of reproduction — they integrate metabolic, photoperiodic, and hormonal signals to gate GnRH release. Kisspeptin-10 provides the upstream hypothalamic trigger for the entire reproductive axis.
Supplies Needed
Reconstitution
Small volume subcutaneous injection
Sterilization
Storage
Store at -20°C; stable 2+ years
Refrigerate at 2–8°C; use within 30 days
This information is provided for educational and research purposes only. Not approved for human consumption by the FDA or any regulatory body. Always consult a qualified medical professional.
Dosing Protocols
Timing: Morning and evening. Very short acting u2014 twice daily mimics pulsatile kisspeptin secretion
↓ Apply these values to the reconstitution calculatorTiming: Morning, midday, and evening. Stack with Gonadorelin for full HPG axis support
↓ Apply these values to the reconstitution calculatorTiming: Every 6u20138 hours. Advanced protocols for hypothalamic hypogonadism; may combine with Clomiphene for additive LH stimulation
↓ Apply these values to the reconstitution calculatorWeekly Timeline
| Week | Expected Effects |
|---|---|
| Week 1 | Increased LH/FSH within hours of dosing; early testosterone elevation; improved energy and libido |
| Week 2 | Sustained testosterone improvement; enhanced sexual function; testicular stimulation maintained |
| Week 4 | HPG axis normalization; improved testosterone levels; fertility markers improving |
| Week 8 | Full HPG axis optimization; maintained reproductive function; hormonal balance |
Reconstitution Calculator
1 unit on U-100 syringe = 0.01 mL · Always label your vial after reconstitution
Injection Technique & Reconstitution
- Wipe vial stopper with alcohol swab
- Draw 2 mL bacteriostatic water
- Inject slowly along vial wall
- Gently swirl until dissolved
- Refrigerate at 2u20138u00b0C
Use our free dosing calculator to get the exact syringe units for your vial size and dose.
Mechanism of Action
Kisspeptin-10 (the C-terminal decapeptide of kisspeptin-54) binds to GPR54 (KISS1R) receptors on hypothalamic GnRH neurons with high affinity. This triggers Gq/11 signaling, phospholipase C activation, IP3-mediated calcium release, and ultimately stimulates a burst of GnRH secretion. The GnRH pulse then triggers LH and FSH release from the pituitary. Kisspeptin neurons in the hypothalamus (particularly in the arcuate nucleus and anteroventral periventricular nucleus) are the master regulators of reproduction — they integrate metabolic, photoperiodic, and hormonal signals to gate GnRH release. Kisspeptin-10 provides the upstream hypothalamic trigger for the entire reproductive axis.
Key Research Papers
Demonstrated that a single IV injection of Kisspeptin-10 in healthy males produced a dose-dependent increase in LH, FSH, and testosterone, confirming its role as an upstream GnRH trigger.
View on PubMed →Confirmed kisspeptin's role in regulating the pulsatile nature of GnRH secretion.
View on PubMed →Stacks Well With
Kisspeptin acts upstream (hypothalamus u2192 triggers GnRH neurons); Gonadorelin acts at the next step (pituitary). Together they provide dual-level HPG axis stimulation.
Sermorelin handles GH axis; Kisspeptin-10 handles reproductive axis u2014 comprehensive hormonal optimization without exogenous hormones
Frequently Asked Questions
Kisspeptin-10 (KP-10) is the shortest and most potent fragment, binding GPR54 with higher affinity per unit than KP-13, KP-14, or KP-54. It is the C-terminal decapeptide that contains the active binding motif (RF-amide).
Only if the underlying cause of low testosterone is hypothalamic (GnRH deficiency), not testicular or pituitary. It restores the natural signal but the gonads must still be able to respond.
Metastin is the full-length kisspeptin-54 product, originally identified as a tumor metastasis suppressor. Kisspeptin-10 is its C-terminal bioactive fragment, which has the same GPR54 binding activity.
Legal Status by Region
This information is provided for educational and research purposes only. Not approved for human consumption by the FDA or any regulatory body. Always consult a qualified medical professional.