TB-500
Also known as: Thymosin Beta-4 fragment · TB4 fragment 17-23 · TB500
TB-500 promotes actin polymerization by sequestering G-actin monomers via its LKKTETQ actin-binding domain, driving cell migration, proliferation, and differentiation. It upregulates metalloproteinases and promotes angiogenesis through VEGF signaling. TB-500 reduces inflammation by downregulating NF-kB and inflammatory cytokines. It accelerates keratinocyte...
What It Is
TB-500 promotes actin polymerization by sequestering G-actin monomers via its LKKTETQ actin-binding domain, driving cell migration, proliferation, and differentiation. It upregulates metalloproteinases and promotes angiogenesis through VEGF signaling. TB-500 reduces inflammation by downregulating NF-kB and inflammatory cytokines. It accelerates keratinocyte and endothelial cell migration to wound sites, mobilizes stem cells and progenitor cells from bone marrow, and exerts cardioprotective effects by promoting cardiomyocyte survival after ischemic injury.
Supplies Needed
Reconstitution solvent
Drawing and injecting larger volumes
Precise dosing for subcutaneous injection
Sterilization
Storage
Store at -20C for up to 2 years. Stable at 2-8C for up to 6 months. Avoid repeated freeze-thaw cycles.
Refrigerate at 2-8C; use within 30 days. Do not freeze.
This information is provided for educational and research purposes only. Not approved for human consumption by the FDA or any regulatory body. Always consult a qualified medical professional.
Dosing Protocols
Timing: Consistent days e.g. Monday and Thursday. Load for 4-6 weeks then reduce to maintenance 2 mg/week
↓ Apply these values to the reconstitution calculatorTiming: Any time of day. Classic loading/maintenance protocol used in research
↓ Apply these values to the reconstitution calculatorTiming: Any time of day. High-dose loading for athletes with significant injuries; always stack with BPC-157
↓ Apply these values to the reconstitution calculatorWeekly Timeline
| Week | Expected Effects |
|---|---|
| Week 1 | Mild anti-inflammatory effect; reduced systemic soreness |
| Week 2 | Improved recovery between training sessions; early reduction in injury pain |
| Week 4 | Significant improvement in chronic injury symptoms; improved flexibility |
| Week 8 | Full systemic regenerative benefit; most acute injuries resolved; chronic conditions substantially improved |
Reconstitution Calculator
1 unit on U-100 syringe = 0.01 mL · Always label your vial after reconstitution
Injection Technique & Reconstitution
- Remove the flip-top cap from the vial
- Swab the rubber stopper with an alcohol swab and allow to dry
- Draw 2 mL bacteriostatic water into a syringe
- Slowly inject the BAC water down the inner wall of the vial - avoid direct spray onto the powder
- Gently swirl until completely dissolved - solution should be clear
- Label with reconstitution date
- Refrigerate at 2-8 degrees C
Use our free mixing calculator to get the exact syringe units for your vial size and dose.
Mechanism of Action
TB-500 promotes actin polymerization by sequestering G-actin monomers via its LKKTETQ actin-binding domain, driving cell migration, proliferation, and differentiation. It upregulates metalloproteinases and promotes angiogenesis through VEGF signaling. TB-500 reduces inflammation by downregulating NF-kB and inflammatory cytokines. It accelerates keratinocyte and endothelial cell migration to wound sites, mobilizes stem cells and progenitor cells from bone marrow, and exerts cardioprotective effects by promoting cardiomyocyte survival after ischemic injury.
Key Research Papers
Demonstrated that Thymosin Beta-4 significantly accelerated wound healing in animal models by promoting keratinocyte migration and angiogenesis.
View on PubMed →Showed cardioprotective effects of Thymosin Beta-4 including promotion of cardiomyocyte survival and cardiac repair after ischemic injury.
View on PubMed →Stacks Well With
The gold standard healing stack. TB-500 provides systemic angiogenesis and cell migration while BPC-157 targets local tissue repair and gut healing. Highly synergistic.
Collagen remodeling synergy - TB-500 drives cell migration and angiogenesis while GHK-Cu stimulates collagen production and tissue remodeling.
Frequently Asked Questions
TB-500 is used for systemic injury recovery, accelerating healing of muscles, tendons, ligaments, and cardiovascular tissue. It is especially popular for injuries that are difficult to treat locally.
TB-500 works systemically through the bloodstream to promote angiogenesis and cell migration throughout the body. BPC-157 is more locally targeted and also has direct GI healing effects. They are commonly stacked together.
A typical protocol involves a loading phase of 2-5 mg twice weekly for 4-6 weeks, followed by a maintenance phase of 2 mg once weekly.
Legal Status by Region
This information is provided for educational and research purposes only. Not approved for human consumption by the FDA or any regulatory body. Always consult a qualified medical professional.