Neuropeptide Vasodilator Immunomodulator

VIP (Vasoactive Intestinal Peptide)

Also known as: Vasoactive Intestinal Peptide · VIP peptide · PHI-27

VIP is a pleiotropic neuropeptide that binds to VPAC1 and VPAC2 receptors (vasoactive intestinal peptide receptors 1 and 2), which are class B GPCRs coupling primarily to Gs protein → adenylyl cyclase → cAMP elevation. VPAC1 is expressed on immune...

Half-life ~2 minutes in plasma (rapidly degraded by plasma peptidases); intranasal delivery extends CNS exposure
Mol. weight 3326.8 Da
Typical cycle 8 weeks
Legal (US) Research chemical u2014 unscheduled,...
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What It Is

VIP is a pleiotropic neuropeptide that binds to VPAC1 and VPAC2 receptors (vasoactive intestinal peptide receptors 1 and 2), which are class B GPCRs coupling primarily to Gs protein → adenylyl cyclase → cAMP elevation. VPAC1 is expressed on immune cells, smooth muscle, epithelium, and CNS neurons. VPAC2 is expressed on smooth muscle, CNS, and immune cells. VIP causes vasodilation, bronchodilation, and smooth muscle relaxation. In the immune system, it suppresses Th1 inflammation and promotes Th2 responses, shifting away from inflammatory cytokines (TNF-α, IL-6, IL-12) toward anti-inflammatory mediators (IL-10, TGF-β). It is neuroprotective, supports circadian rhythm via suprachiasmatic nucleus signaling, and is critical in the gut-brain axis. VIP deficiency is implicated in chronic inflammatory conditions, POTS, and mast cell activation syndrome (MCAS).

Supplies Needed

Bacteriostatic Water or Sterile Saline 10 mL

Reconstitution

Nasal atomizer device

Intranasal delivery

Insulin syringes 0.5 mL U-100

Subcutaneous injection

Alcohol swabs

Sterilization

Storage

Lyophilized (powder)

Store at -20°C; stable 2+ years; highly sensitive to heat

Reconstituted (liquid)

Refrigerate at 2–8°C; use within 14 days; more labile than most peptides

⚠ Disclaimer

This information is provided for educational and research purposes only. Not approved for human consumption by the FDA or any regulatory body. Always consult a qualified medical professional.

Dosing Protocols

Dose50u2013100 mcg
FrequencyOnce to twice daily
Cycle Length4 weeks
RouteIntranasal

Timing: Morning, intranasally. Intranasal is preferred for neurological and CNS applications; prepare in sterile saline for nasal spray

↓ Apply these values to the reconstitution calculator
Dose100u2013200 mcg
FrequencyTwice daily
Cycle Length8 weeks
RouteIntranasal or subcutaneous

Timing: Morning and evening. For MCAS/POTS/inflammatory conditions; intranasal is standard research route

↓ Apply these values to the reconstitution calculator
Dose200u2013400 mcg
FrequencyTwice to three times daily
Cycle Length12 weeks
RouteIntranasal or subcutaneous

Timing: Morning, midday, evening. Advanced inflammatory condition management; IV used in clinical research settings

↓ Apply these values to the reconstitution calculator

Weekly Timeline

Week Expected Effects
Week 1 Reduced inflammatory symptoms; improved GI motility; vasodilation effects (may notice warmth/flushing)
Week 2 Anti-inflammatory benefits developing; improved circulation; reduced mast cell reactivity in MCAS patients
Week 4 Sustained immune modulation; improved GI health; reduced MCAS/POTS symptoms in affected users
Week 8 Comprehensive anti-inflammatory remodeling; improved autonomic regulation; reduced baseline inflammation

Reconstitution Calculator

Concentration
Draw to (units)
Draw to (mL)

1 unit on U-100 syringe = 0.01 mL  ·  Always label your vial after reconstitution

Injection Technique & Reconstitution

  1. Wipe vial stopper with alcohol swab
  2. Draw 2 mL sterile saline or bacteriostatic water
  3. Inject slowly along vial wall
  4. Gently swirl until dissolved
  5. For intranasal: transfer to nasal atomizer; for injection: use insulin syringe
  6. Refrigerate at 2u20138u00b0C; protect from light
Calculate your VIP (Vasoactive Intestinal Peptide) dose

Use our free peptide calculator to get the exact syringe units for your vial size and dose.

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Mechanism of Action

VIP is a pleiotropic neuropeptide that binds to VPAC1 and VPAC2 receptors (vasoactive intestinal peptide receptors 1 and 2), which are class B GPCRs coupling primarily to Gs protein → adenylyl cyclase → cAMP elevation. VPAC1 is expressed on immune cells, smooth muscle, epithelium, and CNS neurons. VPAC2 is expressed on smooth muscle, CNS, and immune cells. VIP causes vasodilation, bronchodilation, and smooth muscle relaxation. In the immune system, it suppresses Th1 inflammation and promotes Th2 responses, shifting away from inflammatory cytokines (TNF-α, IL-6, IL-12) toward anti-inflammatory mediators (IL-10, TGF-β). It is neuroprotective, supports circadian rhythm via suprachiasmatic nucleus signaling, and is critical in the gut-brain axis. VIP deficiency is implicated in chronic inflammatory conditions, POTS, and mast cell activation syndrome (MCAS).

Key Research Papers

Vasoactive intestinal peptide: a neuropeptide with pleiotropic immune functions 2007

Comprehensive review of VIP's immunomodulatory mechanisms, showing its role in suppressing Th1 inflammation and promoting tolerance.

View on PubMed →
Intranasal VIP improves social behavior deficits and serotonin transporter reductions in a mouse model of autism 2014

Demonstrated intranasal VIP delivery improved social behavior deficits and brain serotonin function in autism mouse models.

View on PubMed →

Stacks Well With

BPC-157

BPC-157 heals GI mucosal damage locally; VIP regulates GI motility and inflammation systemically u2014 powerful gut healing stack for IBD, SIBO, leaky gut

KPV

KPV is anti-inflammatory via alpha-MSH pathway; VIP is anti-inflammatory via cAMP/VPAC pathway u2014 dual anti-inflammatory approach for MCAS, IBD, chronic inflammation

Selank

Selank reduces anxiety via GABAergic modulation; VIP is neuroprotective and anti-inflammatory u2014 synergistic neuro-calming stack

Frequently Asked Questions